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PCR Primers for Terrestrial Isopoda (Oniscidea)


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Molecular Phylogenetic Analysis References in the Database

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Beck M.L., Price J.O.  (1981)
Genetic variation in the terrestrial isopod, Armadillidium vulgare.
Journal of Heredity, 72: 15–18.

Beck M.L., Price J.O.  (1985)
Genetic variation and differentiation in Armadillidium vulgare (Isopoda: Oniscoidea)
Genetica, 66: 169–171.

Becking T., Giraud I., Raimond M., Moumen B., Chandler C., Cordaux R., Gilbert C.  (2017)
Diversity and evolution of sex determination systems in terrestrial isopods.
Scientific Reports, 7: 1–39.

Bedek J., Taiti S., Bilandžija H., Ristori E., Baratti M.  (2019)
Molecular and taxonomic analyses in troglobiotic Alpioniscus (Illyrionethes) species from the Dinaric Karst (Isopoda: Trichoniscidae).
Zoological Journal of the Linnean Society, 187: 539–584.

Cooper S., Saint K., Taiti S., Austin A., Humphreys W.  (2008)
Subterranean archipelago; mitochondrial DNA phylogeography of stygobitic isopods (Oniscidea: Haploniscus) from the Yilgarn region of Western Australia.
Invertebrate Systematics, 22: 195–203.

Delhoumi M., Zaabar W., Bouslama M.F., Zayani D., Achouri M.S.  (2019)
High level of genetic variation in mitochondrial 16S rDNA among populations of Porcellionides pruinosus (Brandt, 1833) (Crustacea: Isopoda: Oniscidea) in Tunisia.
The European Zoological Journal, 86: 1–10.

Dimitriou A., Taiti S., Sfenthourakis S.  (2019)
Genetic evidence against monophyly of Oniscidea implies a need to revise scenarios for the origin of terrestrial isopods.
Scientific Reports, 9: 18508.

Doublet V., Raimond R., Grandjean F, Lafitte A, Souty-Grosset C., Marcadé I.  (2012)
Widespread atypical mitochondrial DNA structure in isopods (Crustacea, Peracarida) related to a constitutive heteroplasmy in terrestrial species.
Genome, 55: 234–244.

Garthwaite R.L., Lawson R., Sassaman C.  (1995)
Population genetics of Armadillidium vulgare in Europe and North America.
In: Terrestrial Isopod Biology (Alikhan M.A. Ed.), pp.145–199, A.A. Balkema.

Gentile G., Allegrucci G.  (1999)
Geographic variation and genetic relationships in populations of the Androniscus dentiger complex from Central Italy (Isopoda, Oniscidea, Trichoniscidae).
International Journal of Speleology, 26: 47–61.

Gentile G., Campanaro A., Carosi M., Sbordoni V., Argano R.  (2010)
Phylogeography of Helleria brevicornis Ebner 1868 (Crustacea, Oniscidea): Old and recent differentiations of an ancient lineage.
Molecular Phylogenetics and Evolution, 54: 640–646.

Gentile G., Sbordoni V.  (1998)
Indirect methods to estimate gene flow in cave and surface populations of Androniscia dentiger (Isopoda: Oniscidea).
Evolution, 52: 432–442.

Greenan T., Griffiths C., Santamaria C.  (2018)
Molecular approaches uncover cryptic diversity in intertidal Ligia isopods (Crustacea, Isopoda, Ligiidae) across the southern Africa coastline.
PeerJ, 6: e4658.

Hurtado L., Lee E., Mateos M., Taiti S.  (2014)
Global Diversification at the Harsh Sea-Land Interface: Mitochondrial Phylogeny of the Supralittoral Isopod Genus Tylos (Tylidae, Oniscidea).
PLoS ONE, 9: e94081.

Hurtado L., Mateos M., Santamaria C.  (2010)
Phylogeography of supralittoral rocky intertidal Ligia Isopods in the Pacific region from central California to central Mexico.
PLoS ONE, 5: e11633.

Hurtado L., Mateos M., Wang C., Santamaria C., Jung J., Khalaji-Pirbalouty V., Kim W.  (2018)
Out of Asia: mitochondrial evolutionary history of the globally introduced supralittoral isopod Ligia exotica.
PeerJ, 6: e4337.

Javidkar M., Cooper S.J.B., King R.A., Humphreys W.F., Bertozzi T., Stevens M.I., Austin A.D.  (2016)
Molecular systematics and biodiversity of oniscidean isopods in the groundwater calcretes of central Western Australia.
Molecular Phylogenetics and Evolution, 104: 83–98.

Jung J., Eo H.-S., Rho H.S., Kim W.  (2008)
Two genetic lineages of sea slaters, Ligia (Crustacea: Isopoda) in South Korea: a population genetic approach.
Molecules and Cells, 25: 523–530.

Karasawa S.  (2016)
Eleven nominal species of Burmoniscus are junior synonyms of B. kathmandius (Schmalfuss, 1983) (Crustacea, Isopoda, Oniscidea).
ZooKeys, 607: 1–24.

Karasawa S., Goto K.  (2014)
Burmoniscus kitadaitoensis Nunomura, 2009 (Crustacea, Isopoda, Oniscidea) from southern Japan, a junior synonym of B. meeusei (Holthuis, 1947).
ZooKeys, 386: 21–28.

Karasawa S., Honda M.  (2012)
Taxonomic study of the Burmoniscus ocellatus complex (Crustacea, Isopoda, Oniscidea) in Japan shows genetic diversification in the southern Ryukyus, southwestern Japan.
Zoological Science, 29: 527–537.

Karasawa S., Iwasaki T., Nakajima J.  (2017)
DNA barcoding identified the exotic terrestrial isopod Porcellio scaber (Crustacea, Isopoda, Oniscidea) on the Kyushu mainland, western Japan.
Journal of Field Science, 15: 29–34.

Karasawa S., Kanazawa Y., Kubota K.  (2014)
Redefinitions of Spherillo obscurus (Budde-Lund, 1885) and S. dorsalis (Iwamoto, 1943) (Crustacea: Oniscidea: Armadillidae), with DNA markers for identification.
Edaphologia, 93: 11–27.

Klossa-Kilia E., Kilias G., Sfenthourakis S.  (2005)
Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RELP analysis of mtDNA segments.
Contributions to Zoology, 74: 255–264.

Klossa-Kilia E., Kilias G., Tryfonopoulos G., Koukou K., Sfenthourakis Parmakelis A.  (2006)
Molecular phylogeny of the Greek populations of the genus Ligidium (Isopoda, Oniscidea) using three mtDNA gene segments.
Zoologica Scripta, 35: 459–472.

Niikura M., Honda M., Yahata K.  (2015)
Phylogeography of semiterrestrial isopod, Tylos granuliferus, on east Asian coasts.
Zoological Science, 32: 105–113.

伊谷結  (1999)
ミトコンドリアDNAに基づいた日本産フナムシ属の系統解析.
昆虫と自然, 34: 39–41.

伊谷結  (2000)
分子系統解析に基づく日本産フナムシ類 (等脚目: 甲殻亜門) の系統生物地理.
月刊海洋, 32: 246–251.
Not included

Kilpert F. (2010) Molecular markers of the mitochondrial genomes of isopoda and implications on the phylogeny of peracarida (crustacea: malacostraca). Doctoral thesis. [including many primers, but the pairs were unclear]

Kilpert F., Podsiadlowski L. (2006) The complete mitochondrial genome of the common sea slater, Ligia oceanica (Crustacea, Isopoda) bears a novel gene order and unusual control region features. BMC Genomics, 7: 241. [including many primers, but the pairs were unclear]

Kilpert F., Held C., Padsiadlowski L. (2012) Multiple rearrangements in mitochondrial genomes of Isopoda and phylogenetic implications. Molecular Phylogenetics and Evolution, 64: 106–117. [including many primers, but the pairs were unclear]


Primer Name and Sequence in the Database

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12S aih1: GGATTAGATACCCTACTATTTATAAAC

12S bih1: GACGGGCGATGTGTACTTATTC

12SCRF: GAGAGTGACGGGCGATATGT

12SCRR: AAACCAGGATTAGATACCCTATTAT

12Sai: AAACTAGGATTAGATACCCTATTAT

12Sbi: AAGAGCGACGGGCGATGTGT

16S F: CGGTTTGAACTCAAATCATGTG

16S R: GCCTGTTTAACAAAAACATC

16S Tyhe-F: ATATTGACTGTGCTAAGGTAGC

16S Tyhe-R: CTTAATCCAACATCGAGGTC

16S tyhefor: ATATTGACTGTGCTAAGGTAGC

16S tyherev: CTTAATCCAACATCGAGGTC

16S2: GCGACCTCGATGTTGGATTAA

16SAR-SF: CGCCTGTTTATCAAAAACAT

16SBR-SF: CCGGTTTGAACTCAGATCATG

16SH2: AGATAGAAACCAACCTGG

16Sar: CGCCTGTTTATCAAAAACAT

16Sar-int-sf: GCCGCAGTATHCTRACTGTGCT

16Sbr: CCGGTCTGAACTCAGATCACGT

16s SAR: CGCCTGTTTATCAAAAACAT

16s SBR: CCGGTCTGAACTCAGATCACGT

16s TA: CTGTGCTAAGGTAGCRTAAT

16s TB: TTAARRGTCGAACAGAC

18Aimod: noData

18S614r: TCCAAC TACGAGCTTTTTAACC

18Sforward: TACCTGGTTGATCCTGCCAG

18s1.2F: TGCTTGTCTCAAAGATTAAGC

18sb5.0: TAACCGCAACAACTTTAAT

28sa: GACCCGTCTTGAAACACGGA

28sb: TCGGAAGGAACCAGCTACTA

28srD1.2a: CCCSSGTAATTTAAGCATATTA

28srd4.2b: CCTTGGTCCGTGTTTCAAGACGG

700 R: CGCGGCTGCTGGCACCAGCAC

C1-J-2329 (alias K525): ACTGTAAATATATGATGAGCTCA

COI-F: TCWACNAAYCAYAARGAYATTGG

COI-R: ACYTCNGGRTGNCCRAARARYCA

COIarch1: GGTCAACAAATCATAAAGAYATYGG

COIarch2: TAAACTTCAGGGTGACCAAAAAATCA

CYTOB2_F: AATTGGAAGTGARGAAGCAGCA

CYTOB2_R: TTACRGACTGAACWGATGARGCW

Crust-12f: CAGCAKYCGCGGTTAKAC

Crust-12r: ACACCTACTWTGTTACGACTTATCTC

Cytb144F: TGAGSNCARATGTCNTWYTG

Cytb151F: TGTGGRGCNACYGTWATYACTAA

Cytb270R: AANAGGAARTAYCAYTCNGGYTG

Cytb272R: GCRAANAGRAARTACCAYTC

D-forward: TGTGATGCCCTTAGA

D-reverse: TCTAAGGGCATCACA

D3A: GACCCGTCTTGAAACACGGA

D3B: TCGGAAGGAACCAGCTACTA

FLO-HCO: GGRTGYCCAAARAATCA

G2281: GSGATGCCGCGTWTGGGAGN

G2282: TTCACCGTCBVAGAGGCCGT

G2328: GTGCCACYGCCAAACCT

G2329: CCATRCCCCAACCTSCTGT

G2340: GATCGTGTWTAYGAAGTYGGAAG

G2341: TCAAGAGCRGTACARWAGTTTTC

GROW42_F: YTTRGGKCTTGGAGCMCTGT

GROW42_R: AGGACCTCCCCARCTCAAGA

H3AF: ATGGCTCGTACCAAGCAGACVGC

H3AR: ATATCCTTRGGCATRATRGTGAC

H7005mod1: ARTGNGCANCANCRTARTANGTRTCRTG

HCO2198: TAAACTTCAGGGTGACCAAAAAATCA

HCO2198_t1: CAGGAAACAGCTATGACTAAACTTCAGGGTGACCAAAAAATCA

I-forward: AAACTTAAAGGAATTGACGGA

I-reverse: TCCGTCAATTCCTTTAAGTTT

INN2_F: GCAGCTCTTTYTGGTKGACAG

INN2_R: AAGCGTGAAGCCCTTCTTTG

ISOPOD F1: ATTCTACCAACCATAAGGATATTGG

ISOPOD R1: TCAAAAAAAGATGTATTTAAGTTTCG

LCO1490: GGTCAACAAATCATAAAGATATTGG

LCO1490: GGTCAACAAATCATAAAGATATTGGGG

LCO1490_t1: TGTAAAACGACGGCCAGTGGTCAACAAATCATAAAGATATTGG

LSUF: CGCCTGTTTAACAAAGACAT

LSUR: TCGGTCTGAACTGAACTCAGATCACGT

M13FLCO-1490: TGTAAAACGACGGCCAGTGGTCAACAAATCATAAAGATATTGG

M13RHCO-2198: CAGGAAACAGCTATGACTAAACTTCAGGGTGACCAAAAAATCA

M876: CARTTYCCAAAMCCNCCAATTA

M877: AATTTTATTACAACTRTRATHAATATRCG

METH24_F: TCTTGTTCGCAGCCAAARAYT

METH24_R: TGGGCATGGATAMCCRTTAAG

N4: GGAGCTTCAACATGAGCTTT

N4-specific2: CYCCRWCYTGGAGKCWYTCSGGY

NADH19_F: GATTCCWCCTCTGCTYTGGT

NADH19_R: GAYTCTCGCTTGACCCAATCM

NAK 638 R: noData

NAK for-b: noData

NAK for-b: ATGACAGTTGCTCATATGTGGTT

NAK rev2: noData

NAK rev2: ATAGGGTGATCTCCAGTRACCAT

NaKFb: noData

NaKR2: noData

PEPCK 545 R: noData

PEPCKfor: noData

PEPCKrev: noData

PROT44_F: AAGCAGGRATTWTGGSTYTGG

PROT44_R: RATGGCATTTGCAGTRGTWCG

RIB46_F: GAACCWTATGTGCCYCCMGA

RIB46_R: GKAYACGCCACAACCCRTA

UBI4_F: GAAACTTCTGGGGATTGAAGCAC

UBI4_R: ACCGGYWGGCATTTGATTGA

VATP10_F: GATCCAGCGTCCRCTTCARG

VATP10_R: CTCCRCAACCCACGTACACA

XAA6_F: GCTGAAAAAGYRTGGGTTGGA

XAA6_R: AAKCMTGRTCTGGGATATAAGM

crust-12Sf: CAGCAKYCGCGGTTAKAC

crust-12Sr: ACACCTACTWTGTTACGACTTATCTC

heCOIfor: TCCTTTAGCTTCAAATATTGC

heCOIrev: CATAATGGAAATGAGCTACAAC

noData: noData


Directed by Shigenori Karasawa (Tottori University: dojyoudoubutu (a) gmail.com)